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| Reactivity: | H,M,R & R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | eRF1 rabbit polyclonal antibody |
Gene Name: | Eukaryotic peptide chain release factor subunit 1 |
Synonyms: | Eukaryotic release factor 1, eRF1, Etf1, ERF, RF1 |
Immunogen: | Recombinant protein corresponding to Mouse eRF1 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. | 49 kDa |
Observed MW. | 50 kDa |
Uniprot ID: | P62495, Q8BWY3, Q5U2Q7 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | liver, kidney, testis |
IHC/IF | Rat | 1: 750-1: 4000 | brain, testis |
Background
This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in coding and non-coding transcript variants. Pseudogenes of this gene are found on chromosomes 5, 7 and X.
Images
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Western blot analysis of erf1 (GB111098) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded rat brain using erf1 (GB111098) at dilution of 1: 2400 (400x lens) |
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Immunohistochemistry of paraffin embedded rat testis using erf1 (GB111098) at dilution of 1: 2400 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
