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Anti-splicing factor 1 Rabbit pAb (100 μl)
| Reactivity: | H,M |
| Applications: | IHC |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | splicing factor 1 rabbit polyclonal |
Gene Name: | Splicing factor 1 |
Synonyms: | BBP, mBBP, CW17, D11S636, ZFM1, Transcription factor ZFM1, Splicing factor 1, Zinc finger gene in MEN1 locus, Zinc finger protein 162, ZNF162, Sf1, Zfm1, ZCCHC25 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse splicing factor 1 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
IHC | Human, Mouse | 1: 200-1: 400 | placenta, ovary, spleen, testis |
Background
This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.
Images
| Immunohistochemistry analysis of paraffin-embedded human placenta using SF1 (GB113738) at dilution of 1: 400 |
| Immunohistochemistry analysis of paraffin-embedded mouse ovary using SF1 (GB113738) at dilution of 1: 400 |
| Immunohistochemistry analysis of paraffin-embedded mouse spleen using SF1 (GB113738) at dilution of 1: 400 |
| Immunohistochemistry analysis of paraffin-embedded mouse testis using SF1 (GB113738) at dilution of 1: 400 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
