Anti-Slow Skeletal Myosin Heavy chain Mouse mAb (100 μl)

Myosin-7

Synonyms:

Myosin heavy chain 7, Myosin heavy chain slow isoform, MyHC-slow, MyHC-beta, MYH7, MYHCB, CMD1S, SPMM, SPMD, MPD1, Beta myosin heavy chain

Immunogen:

KLH conjugated Synthetic peptide corresponding to Human MYH7

Uniprot ID:

P12883

Isotype:

IgG1,k

Purity:

Affinity purification

Subcellular location:

Cytoplasm

IHC/IF

Mouse, Rat

1: 500-1: 1000

gastrocnemius, soleus muscle, back muscles

IF analysis of Slow Skeletal Myosin Heavy chain(GB121857) green.
Sample: Mouse gastrocnemius, 4% PFA (G1101) 12-24h.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 800, 4°C overnight.
Secondary antibody: 488 conjugated Goat Anti-Mouse IgG (H+L) (GB25301), 1: 400, RT, 1h.

IF analysis of Slow Skeletal Myosin Heavy chain(GB121857) green.
Sample: Mouse soleus muscle, 4% PFA (G1101) 12-24h.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 800, 4°C overnight.
Secondary antibody: 488 conjugated Goat Anti-Mouse IgG (H+L) (GB25301), 1: 400, RT, 1h.

IF analysis of Slow Skeletal Myosin Heavy chain(GB121857) green.
Sample: Rat back muscles, 4% PFA (G1101) 12-24h.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 800, 4°C overnight.
Secondary antibody: 488 conjugated Goat Anti-Mouse IgG (H+L) (GB25301), 1: 400, RT, 1h.