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Anti-TSG101 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & M |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Tumor susceptibility gene 101 protein |
Synonyms: | TSG101, TSG10, VPS23, tumor susceptibility 101 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse TSG101(N-terminal) |
Uniprot ID: | Q61187 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 44 kDa / 46 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | liver, lung, brain |
ICC/IF | Mouse | 1: 50-1: 200 | NIH-3T3 |
Background
The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation.Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression
Images
| WB analysis of TSG101(GB11618). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
|
ICC analysis of TSG101(GB11618)red. Sample: NIH-3T3, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 50, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
