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Recombinant Anti-TTF1 (Thyroid transcription factor 1) Mouse mAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Homeobox protein Nkx-2.1 |
Synonyms: | Homeobox protein NK-2 homolog A,Thyroid nuclear factor 1,Thyroid transcription factor 1 (TTF-1),NKX2-1,NKX2A, TITF1, TTF1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human TTF-1 |
Uniprot ID: | P43699 |
Isotype: | IgG1 |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | lung, thyroid gland |
Background
Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1
Images
| IHC analysis of TTF1(Thyroid transcription factor 1)(GB152168). Sample: Human lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
| IHC analysis of TTF1(Thyroid transcription factor 1)(GB152168). Sample: Mouse lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
| IHC analysis of TTF1(Thyroid transcription factor 1)(GB152168). Sample: Rat lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
| IHC analysis of TTF1(Thyroid transcription factor 1)(GB152168). Sample: Rat thyroid gland, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
