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Recombinant Anti-Tartrate Resistant Acid Phosphatase Mouse mAb (100 μl)
| Reactivity: | H,M & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Tartrate-resistant acid phosphatase type 5 |
Synonyms: | ACP5, HPAP, SPENCDI, TRAP, TRACP5a, TRACP5b, TrATPase, acid phosphatase 5, tartrate resistant |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse TRAP/CD40L |
Uniprot ID: | Q05117 |
Isotype: | IgG1 |
Purity: | Affinity purification |
Subcellular location: | Lysosome |
Predicted MW. / Observed MW. | 37 kDa / 37 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
WB | Human, Mouse | 1: 1000-1: 2000 | HeLa, A549, RAW 264.7 |
IHC/IF | Mouse, Rat | 1: 250-1: 1000 | bone |
Background
May play a role in the process of bone resorption. The osteoclastic trap acts on nucleotide tri- and diphosphates with higher affinity, compared with other substrates.
Images
| WB analysis of Tartrate Resistant Acid Phosphatase(GB15416). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h. |
| IHC analysis of Tartrate Resistant Acid Phosphatase(GB15416). Sample: Mouse bone, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203), 98°C, 20min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min. |
| IHC analysis of Tartrate Resistant Acid Phosphatase(GB15416). Sample: Mouse bone, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203), 98°C, 20min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min. |
| IHC analysis of Tartrate Resistant Acid Phosphatase(GB15416). Sample: Rat bone, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203), 98°C, 20min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
