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Anti-Topoisomerase III alpha Rabbit pAb (100 μl)
| Reactivity: | H |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Topoisomerase III alpha rabbit polyclonal antibody |
Gene Name: | DNA topoisomerase 3-alpha |
Synonyms: | DNA topoisomerase III alpha, TOP3A, TOP3, ZGRF7, topo III-alpha, zinc finger, GRF-type containing 7, topoisomerase III alpha |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Topoisomerase III alpha |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 112 kDa |
Observed MW. | 112 kDa |
Uniprot ID: | Q13472 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human | 1: 500-1: 1000 | HeLa, HEK293, K562 |
Background
DNA topoisomerase 3-alpha (TOP3A) is an essential component of the RMI complex, a complex involves in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. It releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils.
Images
| Western blot analysis of TOP3A (GB111889) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
