Reactivity: | H,M,R & M,R |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | Tuba3a rabbit polyclonal antibody |
Gene Name: | Tubulin alpha-3 chain |
Synonyms: | Alpha-tubulin 3/7, Alpha-tubulin isotype M-alpha-3/7, Tubulin alpha-3/alpha-7 chain, Tuba3a, Tuba3, Tuba3b, Tuba7 |
Immunogen: | Recombinant protein corresponding to Mouse Tuba3a |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Cytoskeleton |
Predicted MW. | 50 kDa |
Observed MW. | 50 kDa |
Uniprot ID: | P05214, Q68FR8 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | brain, testis, kidney |
IHC/IF | Mouse, Rat | 1: 600-1: 1200 | corpus striatum, hippocampus, testis, uterus |
Background
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Images
Western blot analysis of Tuba3a (GB112245) at dilution of 1: 500 | |
Immunofluorescence of paraffin embedded mouse corpus striatum using Tuba3a (GB112245) at dilution of 1: 600 (400x lens) | |
Immunofluorescence of paraffin embedded mouse uterus using Tuba3a (GB112245) at dilution of 1: 600 (450x lens) | |
Immunofluorescence of paraffin embedded rat hippocampus using Tuba3a (GB112245) at dilution of 1: 600 (400x lens | |
Immunofluorescence of paraffin embedded rat testis using Tuba3a (GB112245) at dilution of 1: 600 (350x lens) |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |