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Anti-Ubiquitin Rabbit pAb (100 μl)
| Reactivity: | H & M |
| Applications: | IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | ubiquition |
Synonyms: | Polyubiquitin B, UBB, ubiquitin, ubiquitin B |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human Ubiquitin |
Uniprot ID: | P62987, P0CG48, P0CG47, P62979 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm,Nucleus,Mitochondrion outer membrane,Peripheral membrane protein |
Predicted MW. / Observed MW. | 8 kDa / ≥8 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | 293-T, NIH/3T3, PC-12 |
IHC/IF | Human, Mouse, Rat | 1: 1500-1: 3000 | kindey, heart |
Background
This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants.
Images
| WB analysis of Ubiquitin（GB115700). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| WB analysis of Ubiquitin（GB115700). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| IHC analysis of Ubiquitin(GB115700). Sample: Human kindey, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L),Ready to use(G1302), RT, 20min. |
| IHC analysis of Ubiquitin(GB115700). Sample: Human lung cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L),Ready to use(G1302), RT, 20min. |
| IHC analysis of Ubiquitin(GB115700). Sample: Mouse heart, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L),Ready to use(G1302), RT, 20min. |
| IHC analysis of Ubiquitin(GB115700). Sample: Mouse liver, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L),Ready to use(G1302), RT, 20min. |
| IHC analysis of Ubiquitin(GB115700). Sample: Rat brain, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L),Ready to use(G1302), RT, 20min. |
| IHC analysis of Ubiquitin(GB115700). Sample: Rat heart, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L),Ready to use(G1302), RT, 20min. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
