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Anti-Villin Mouse mAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Villin-1 |
Synonyms: | VIL1, VIL, Villin 1, D2S1471 |
Immunogen: | Recombinant protein corresponding to Mouse Villin |
Uniprot ID: | Q62468 |
Isotype: | IgG2b,κ |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. / Observed MW. | 93 kDa / 95 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | small intestine, kidney, colon |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 2000 | colon, ileum, jejunum |
Background
Villin 1 (VIL1) is a 95-kd F-actin bundling and severing protein and its expression is restricted to epithelial cells with a brush border, like epithelial cells of the intestinal mucosa, gall bladder, renal proximal tubules and ductuli efferentes of the testis. VIL1 has been reported to be an epithelial cell-specific anti-apoptotic protein, and to have an important function in regulating actin dynamics, cell morphology, epithelial-to-mesenchymal transitions, cell migration and cell survival. In addition, VIL1 is a useful diagnostic marker for of various cancer, like cervical and endometrial adenocarcinomas, renal cell carcinoma.
Images
| Western blot analysis of Villin (GB121209) at dilution of 1: 1000 |
 | Immunohistochemistry of paraffin embedded human colon using villin (GB121209) at dilution of 1: 1000 (400x lens) |
 | Immunohistochemistry of paraffin embedded human small intestine using villin (GB121209) at dilution of 1: 1000 (400x lens) |
 | Immunohistochemistry of paraffin embedded mouse colon using villin (GB121209) at dilution of 1: 2000 (400x lens) |
 | Immunohistochemistry of paraffin embedded rat ileum using villin (GB121209) at dilution of 1: 500 (400x lens) |
 | Immunohistochemistry of paraffin embedded rat jejunum using villin (GB121209) at dilution of 1: 2000 (400x lens) |
 | Immunofluorescence of paraffin embedded mouse jejunum using villin (GB121209) at dilution of 1: 500 (400x lens) |
 | Immunofluorescence of paraffin embedded mouse kidney using villin (GB121209) at dilution of 1: 500 (400x lens) |
| Immunofluorescence of paraffin embedded mouse stomach using villin (GB121209) at dilution of 1: 500 (400x lens) |
| Immunofluorescence of paraffin embedded rat colon using villin (GB121209) at dilution of 1: 500 (400x lens) |
| Immunofluorescence of paraffin embedded rat liver using villin (GB121209) at dilution of 1: 500 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
