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Anti-Vimentin Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R & H,M |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Vimentin rabbit polyclonal antibody |
Gene Name: | Vimentin |
Synonyms: | VIM, CTRCT30, HEL113, vimentin |
Uniprot ID: | P08670, P20152, P31000 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse vimentin |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. | 57 kDa |
Observed MW. | 57 kDa |
reactivity （External validation） | Pelodiscus sinensis |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | lung |
IHC/IF | Human, Mouse, Rat | 1: 200-1: 1000 | colon, lung cancer, ovarian cancer |
ICC/IF | Human, Mouse | 1: 200-1: 1000 | HeLa cell, NIH/3T3 cell |
Background
Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all metazoan cells as well as bacteria. IF, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression.
Images
| Western blot analysis of Vimentin (GB11192) at dilution of 1: 500 |
| Immunohistochemistry of paraffin embedded human colon using vimentin (GB11192) at dilution of 1: 500 (400x lens) |
| Immunohistochemistry of paraffin embedded human breast cancer using vimentin (GB11192) at dilution of 1: 500 (200x lens) |
| Immunohistochemistry of paraffin embedded human breast cancer using vimentin (GB11192) at dilution of 1: 400 (400x lens) |
| Immunofluorescence of paraffin embedded human colon using vimentin (GB11192) at dilution of 1: 500 (200x lens) |
| Immunofluorescence of paraffin embedded human ovarian cancer using vimentin (GB11192) at dilution of 1: 400 (200x lens) |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cell using Vimentin (GB11192) at dilution of 1: 800 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH/3T3 cell using Vimentin (GB11192) at dilution of 1: 800 |
Storage
| Storage | Store at -20 °C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
