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Anti-Wnt3a Rabbit pAb (100 μl)
| Reactivity: | R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Wnt3a rabbit polyclonal |
Gene Name: | Protein Wnt-3a |
Synonyms: | |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human Wnt3a |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | |
Predicted MW. | 42/39 kDa |
Observed MW. | 37 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human | 1: 1000-1: 2000 | 293T |
ICC | Human | 1: 800-1: 1600 | 293T |
Background
WNT3A belongs to wingless-type MMTV integration site family, and abnormal Wnt signalling is often associated with severe human diseases, including cancer, osteoporosis and other degenerative disorders (PMID: 20716463). Wnt3a, was shown to promote trophectoderm formation in embryonic stem cells by inducing Cdx2, one of the critical transcription factors of early trophoblast development, in an LEF-1-dependent manner. Wnt3a may also activate other β-catenin-independent cascades such as ERK or PI3K/AKT signalling, again emphasizing the complexity of the Wnt signalling network.
Images
| Western blot analysis of Wnt3a (GB113750) at dilution of 1: 2000 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed 293T cells using Wnt3a GB113750 at dilution of 1: 1500 Left: 293T cell lysate, transfected with human Wnt3a Right: 293T cell lysate, non-transfected |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
