Reactivity: | H,M,R & M,R |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Gene Name: | Alpha actinin |
Synonyms: | Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, actinin, Non-muscle alpha-actinin-1, ACTN1, actinin 1 smooth muscle, Actn1a, BDPLT15, I79_004057 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human ACTN1 |
Uniprot ID: | P12814 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane, Cytoplasm |
Predicted MW. / Observed MW. | 103 kDa / 103 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | spleen, skeletal muscle, heart |
IHC/IF | Mouse, Rat | 1: 500-1: 1000 | kidney, liver, skeletal muscle |
Background
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.
Images
Western blot analysis of tesk1 (GB11930) at dilution of 1: 1000 | |
Immunohistochemistry analysis of paraffin embedded mouse skin using Alpha-actinin (GB111556) at dilution of 1: 900 | |
Immunohistochemistry analysis of paraffin embedded mouse skeletal muscle using Alpha-actinin (GB111556) at dilution of 1: 450 | |
Immunofluorescence analysis of paraffin embedded mouse heart using Alpha-actinin (GB111556) at dilution of 1: 500 | |
Immunofluorescence analysis of paraffin embedded mouse skeletal muscle using Alpha-actinin (GB111556) at dilution of 1: 500 | |
Immunofluorescence analysis of paraffin embedded mouse skin using Alpha-actinin (GB111556) at dilution of 1: 500 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |