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Recombinant Anti-alpha Tubulin Mouse mAb (100 μl)
| Reactivity: | H,M,R & H,M,R & H,M,R |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal ) |
| Full Name: | mouse monoclonal to alpha Tubulin |
Gene Name: | Tubulin alpha-1A chain |
Synonyms: | TUBA1A, B-ALPHA-1, LIS3, TUBA3, tubulin alpha 1a,TUBA1B, K-ALPHA-1, tubulin alpha 1b |
Isotype: | IgG1 |
Purity: | Affinity purification |
Subcellular location: | Cytoskeleton |
Predicted MW. | 50 kDa |
Observed MW. | 55 kDa |
Uniprot ID: |
Product Usage Information
WB | Human, Mouse, Rat | 1: 3000-1: 6000 | lung |
IHC/IF | Human, Mouse, Rat | 1: 1000-1: 2000 | colon |
ICC/IF | Human, Mouse, Rat | 1: 1000-1: 2000 | HeLa |
Background
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly.
Images
| Western blot analysis of alpha Tubulin (GB15200) at dilution of 1: 6000 |
| Immunohistochemistry analysis of paraformaldehyde-fixed HeLa using alpha Tubulin (GB15200) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat colon using alpha Tubulin (GB15200) at dilution of 1: 2000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
