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Anti-beta Actin Mouse mAb (100 μl)
| Reactivity: | H,M,R & H,M,R & H,M,R |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Actin, cytoplasmic 1 |
Synonyms: | ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin, β-Actin |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse beta-Actin |
Uniprot ID: | P60710 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. / Observed MW. | 42 kDa / 45 kDa |
Applications
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | spleen, kidney, brain |
IHC/IF | Human, Mouse, Rat | 1: 400-1: 2000 | colon, kidney, liver |
ICC/IF | Human, Mouse, Rat | 1: 300-1: 1000 | NIH/3T3, A549 |
Background
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Images
> | WB analysis of beta Actin (GB12001). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 5000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h. |
| Immunohistochemistry of paraffin embedded human colon using actin (GB12001) at dilution of 1: 1000 (200x lens) |
 | Immunohistochemistry of paraffin embedded rat liver using actin (GB12001) at dilution of 1: 1000 (200x lens) |
 | Immunohistochemistry of paraffin embedded mouse stomach using actin (GB12001) at dilution of 1: 1000 (400x lens) |
 | Immunohistochemistry of paraffin embedded rat kidney using actin (GB12001) at dilution of 1: 1000 (400x lens) |
 | Immunohistochemistry of paraffin embedded human liver cancer using actin (GB12001) at dilution of 1: 1000 (200x lens) |
| Immunocytochemistry analysis of 4% paraformaldehyde fixed NIH/3T3 cells using β-Actin (GB12001) at dilution of 1: 1000 |
| Immunocytochemistry analysis of 4% paraformaldehyde fixed HeLa cells using β-Actin (GB12001) at dilution of 1: 1000 |
Storage
>| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
