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Anti-beta Tubulin Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Tubulin beta-5 chain |
Synonyms: |
Tubb5, Beta 5 tubulin, Class I beta tubulin, Tubulin beta class I, TUBB1, TUBB, TUBB5, Tubulin Beta-5 Chain,β tubulin, CDCBM6 |
Immunogen: |
KLH conjugated Synthetic peptide corresponding to Mouse β-Tubulin |
Uniprot ID: |
P99024 |
Isotype: |
IgG |
Purity: |
Affinity purification |
Subcellular location: |
Cytoplasm |
Predicted MW. / Observed MW. |
50 kDa / 55 kDa |
Product Usage Information
Applications |
Species |
Dilution |
Positive Sample |
WB |
Human, Mouse, Rat |
1: 1000-1: 2000 |
brain, lung |
IHC/IF |
Human, Mouse, Rat |
1: 500-1: 1500 |
colon, liver cancer, lung, testis |
Background
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Images
|
Western blot analysis of β-tubulin (GB11017) at dilution of 1: 1000 |
|
Immunohistochemistry of paraffin embedded human colon using beta Tubulin (GB11017) at dilution of 1: 800 (400x lens) |
|
Immunohistochemistry of paraffin embedded human liver cancer using beta Tubulin (GB11017) at dilution of 1: 800 (400x lens) |
|
Immunohistochemistry of paraffin embedded mouse testis using beta Tubulin (GB11017) at dilution of 1: 800 (400x lens) |
|
Immunohistochemistry of paraffin embedded rat testis using beta Tubulin (GB11017) at dilution of 1: 800 (400x lens) |
|
Immunofluorescence of paraffin embedded human colon using beta Tubulin (GB11017) at dilution of 1: 500 (400x lens) |
|
Immunofluorescence of paraffin embedded mouse testis using beta Tubulin (GB11017) at dilution of 1: 500 (300x lens) |
|
Immunofluorescence of paraffin embedded rat lung using beta Tubulin (GB11017) at dilution of 1: 500 (400x lens) |
Storage
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
