Reactivity: | H,M,R |
Applications: | WB |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | eIF4A1 rabbit polyclonal |
Gene Name: | Eukaryotic initiation factor 4A-I |
Synonyms: | Eif4a1, EIF4A1, DDX2A, EIF-4A, EIF4A, eIF-4A-I, eIF4A-I, Eukaryotic translation initiation factor 4A1 |
Immunogen: | Recombinant protein corresponding to Mouse eIF4A1 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 46 kDa |
Observed MW. | 46 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1500 | liver, muscle, brain |
Background
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Images
Western blot analysis of eif4a (GB11719) at dilution of 1: 1000 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |