LiGreen™ Red Loading Dye is used for running DNA samples on agarose gel. The Loading Dye contains two regular dyes (Xylene Cyanol FF and Bromophenol Blue), and a safe, non-carcinogen green fluorescent dye. The Xylene Cyanol FF and Bromophenol Blue are used for monitoring the agarose gel while it is running, and the green fluorescent dye is to replace the toxic carcinogen ethidium bromide (EtBr). During the gel electrophoresis, the agarose gel will reveal a purple band and a blue band. The gel can be directly visualized under LED or UV light without any extra staining steps.
Key Features
• Safer than EB. Shown by Ames test and other tests to be non-mutagentic and non-cytotoxic.
• Stable at room temperature.
• Compatible with standard blue LED and UV transilluminators.
• Use with blue light reduces mutagenesis for gel excision and subsequent cloning.
Component
1. LiGreen™ Red Landing Dye: 2 ml
Storage
Store at -20°C and protect from light.