Mouse Tissue Direct PCR Kit (200 rxns)

The Mouse Tissue Direct PCR Kit adopts a unique packaging design that includes all the reagents for rapid genomic DNA preparation and PCR amplification. It is applicable for the one-step genome DNA purification from mouse tissues (tail, ear and toe) and the subsequent PCR amplification and detection for mouse genotyping. The whole process does not include homogenization, smashing, overnight digestion, organic solvent extraction, ethanol precipitation or column purification steps. Stable results can be obtained with simple and fast operations.The 2× Dir PCR Master Mix provided by this kit is a highly compatible PCR reagent that can efficiently and specifically amplify DNA without the need for removing impurities such as proteins.

This reagent contains an antibody modified hot-start Taq DNA polymerase, dNTPs, MgCl2, buffers, as well as the enhancer, optimizer and stabilizer for PCR reaction. The PCR reaction can be performed by simply adding in roughly extracted template and specific primers. The whole process is fast, simple, sensitive, specific and stable, which is especially suitable for high-throughput screening. The 2× Dir PCR Master Mix contains premix electrophoresis dye, so that the PCR products can be directly sent for electrophoresis detection after the reaction. The 3’ end of the PCR product has a A-tailing, which can be used for TA cloning.
 

Key Features

• Simple and fast: Genomic DNA can be rapidly extracted from mouse tissues in 60 minutes without liquid nitrogen grinding and organic solvent extraction.

• Wide application: It is suitable for one-step extraction of genomic DNA from mouse tail, ear, toe and other tissues.

• High specificity: The Taq polymerase used in this product is an antibody modified hot-start enzyme, with high template and primer affinity and amplification specificity, which is especially suitable for genotyping and transgenic identification.

• Gene detection: The product is easy to operate with reliable results, and it is especially suitable for high-throughput analysis and detection of mouse tissues.
 

Applications

• Mouse genotyping
 

Components

1. Tissue Lysis Buffer: 20 ml
2. Digestive Enzyme: 800 µl
3. 2× Dir PCR MasterMix: 2× 1.25 ml
4. RNase-Free ddH2O: 2× 1 ml
 

Storage

The Tissue Lysis Buffer and Digestive Enzyme should be stored under room temperature (15-25°C ). The 2×Dir PCR Master Mix should be stored at -20°C .

Case Study

Using Mouse Tissue Direct PCR Kit and relevant product from supplier A to amplify 1000 bp, 2000 bp and 3000 bp fragments from mouse tail, mouse ear and rat tail respectively. The result showed that Mouse Tissue Direct PCR Kit has better specificity and success rate.