Your shopping cart is empty!
| Reactivity: | H,M |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Cyclin-dependent kinase inhibitor 1 |
Synonyms: | CDKN1A, CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse p21 |
Uniprot ID: | P39689 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 19 kDa / 20 kDa |
Product Usage Information
WB | Human, Mouse | 1: 300-1: 600 | 293T, MCF7, T47D, NIH3T3 |
Background
May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding.
Images
| Western blot analysis of P21 (GB115313) at dilution of 1:300 |
| Western blot analysis of P21 (GB115313) at dilution of 1:300 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
