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Anti-p27 KIP 1 Rabbit pAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | p27 KIP1 rabbit polyclonal antibody |
Gene Name: | Cyclin-dependent kinase inhibitor 1B |
Synonyms: | CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27KIP1, cyclin-dependent kinase inhibitor 1B, cyclin dependent kinase inhibitor 1B |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse p27 KIP1 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Cytoplasm, Endosome |
Uniprot ID: | P46527, P46414 |
Product Usage Information
IHC/IF | Human, Mouse, Rat | 1: 100-1: 500 | colon, heart, hepatitis |
ICC/IF | Mouse, Rat | 1: 200-1: 800 | HeLa |
Background
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Images
 | Immunohistochemistry of paraffin embedded human stomach using P27 (GB11154) at dilution of 1:400 (400x lens) |
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Immunohistochemistry of paraffin embedded human subcutaneous transplant tumor using P27 (GB11154) at dilution of 1:400 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse Skeletal muscle using P27 (GB11154) at dilution of 1:500 (400x lens) |
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Immunohistochemistry of paraffin embedded rat Skeletal muscle using P27 (GB11154) at dilution of 1:500 (400x lens) |
 | Immunofluorescence of paraffin embedded mouse heart using P27 (GB11154) at dilution of 1:400 (400x lens) |
 | Immunofluorescence of paraffin embedded rat colon using P27 (GB11154) at dilution of 1:400 (400x lens) |
 | Immunofluorescence of paraffin embedded rat hepatitis using P27 (GB11154) at dilution of 1:400 (400x lens) |
 | Immunofluorescence of paraffin embedded rat nephritis using P27 (GB11154) at dilution of 1:400 (400x lens) |
 | Immunofluorescence of paraffin embedded rat pneumonia using P27 (GB11154) at dilution of 1:400 (400x lens) |
| ICC analysis of p27 KIP 1(GB11154)red. Sample: HeLa, 4% PFA (G1101) 20min. Primary antibody: 1: 400, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
