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| Key Features: | • The mix, prepared at 2× reaction concentration, can be directly used for robust and low-template quantitative PCR with high sensitivity, specificity and reliability. • ROX reference dyes are provided for different qPCR instruments. |
| Description: | LiQuant™ Probe qPCR Master Mix is a special reagent designed for probe based qPCR. Utilizing hot-start LiQuant™ DNA polymerase, alone with optimal buffer specially optimized for qPCR, it is capable of significant restraining non-specific amplification as well as enhancing the amplification efficiency, thus is applicable for high-sensitivity qPCR reactions. It is a 2× premixed reagent which is convenient to handle. When using this product for qPCR reactions, reliable standard curve can be obtained in a wide range and accurate qualification of target genes can be achieved with high repeatability and reliability. |
| Component: | 1. LiQuant Probe qPCR Master Mix: 5 ml 2. 50× ROX Reference Dye 1: 200 μl 3. 50× ROX Reference Dye 2: 200 μl |
| Passive Reference Dye: | • Do Not Use ROX Reference Dye. Bio-Rad CFX96™, CFX384™, iCycler iQ™, iQ™5, MyiQ™, MiniOpticon™, Opticon®, Opticon 2, Chromo4™; Cepheid SmartCycler®; Roche Applied Science LightCyclerTM 480r. • Use ROX Reference Dye 1 (final 1×) ABI 7900HT, 7300; StepOnePlus™ • Use ROX Reference Dye 2 (final 1×) ABI 7500, 7500; Stratagene Mx3000P. |
| Storage: | This product should be stored at -20°C protecting from light for 12 months. Repetitive freeze-thaw cycles while using. It is recommended to pre-aliquot the mix into small batches for frequent usage. The quality of this product is guaranteed for 12 months under proper storage conditions. |
| Documents: | Manual MSDS |
