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sgRNA Synthesis
sgRNA (single guide RNA) is known as the command center of the CRISPR/Cas9 gene editing system, which can guide Cas9 to perform targeted editing of DNA. Chemically synthesized sgRNAs usually have low cytotoxicity, and their stability can be enhanced by adding a variety of modifications, thereby reducing off-target effects. Compared with in vitro transcribed sgRNA and plasmid sgRNA, it has the advantages of lower cost, higher editing efficiency, and better batch-to-batch consistency. LifeSct provides sgRNA design and chemical synthesis services. Deliverables are tested by mass spectrometry for sequence accuracy and purified by HPLC. We are committed to providing you with sgRNAs with low toxicity, batch-to-batch stability, and high editing efficiency.
Key Features
• Customized synthesis and flexible synthesis specifications, we can provide large-scale RNA synthesis
• Experienced production and R&D team, mature RNA synthesis and modification technology platform.
• Professional technical support team, providing comprehensive pre-sales guidance and after-sales service.
• Strict quality control standards, ISO9001 quality management system, all products are purified by HPLC.
Online Quote
Please click the button on the right side and login to your account to request a quote.
Service Options (Cat. #: S0015)
| Service | Specifications | Starting Price | Turnaround Time |
| Chemically synthesized sgRNA | 20-100 nt, HPLC, >2 OD | Quote | Starting at 7 days |
sgRNA Libraries
| Service | Description | Starting Price | Turnaround Time |
| sgRNA Design | sgRNA design targeting specific genes according to customer’s requirements | Quote | 1-2 weeks |
| sgRNA Library Construction | sgRNA and control synthesis Constructed the sgRNA to designated vector | Quote | 5-7 weeks |
| Plasmid Preparation | Transfection grade plasmid preparation | Quote | 1 week |
