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The TIANamp Bacteria DNA Kit adopts a highly efficient, DNA-specific adsorption spin column and a unique buffer system for both genomic DNA extraction from Gram-negative and Gram-positive bacteria. The kit can also be used for the extraction of pathogenic bacteria (microorganisms) of food, such as Staphylococcus aureus, cholera sclerotia, hemorrhagic Escherichia coli O157:H7, Listeria monocytogenes, Salmonella, Enterobacter sakazakii, etc. 10-40 μl of pure genomic DNA can be rapidly extracted and purified from 1-5 ml of bacterial culture medium, and the obtained genomic DNA can be directly used in molecular biology experiments such as PCR template, restriction enzyme digestion and Southern blot.
Key Features
• Simple and fast: High purity genomic DNA of Gram-negative bacteria can be obtained within 1 hour.
• Excellent quality: The purified DNA can be directly used in downstream molecular experiments such as PCR, restriction endonuclease digestion, Southern blotting, etc.
Applications
Genome extraction of Gram-negative bacteria, Gram-positive bacteria and pathogenic bacteria in food.
Specificationss
Components
1. Buffer GA: 15 ml
2. Buffer GB: 15 ml
3. Buffer GD: 13 ml
4. Buffer PW: 15 ml
5. Buffer TE: 15 ml
6. Proteinase K: 1 ml
7. Spin Columns CB3: 50
8. Collection Tubes 2 ml: 60
Storage
Store at room temperature.
Case Study
Extraction of bacterial genomic DNA from various sources using TIANamp Bacteria DNA Kit.
