Your shopping cart is empty!
LiGreen™ Ultra Nucleic Acid Gel Stain is a ultra sensitive green fluorescent stain for detecting nucleic acids in agarose and polyacrylamide gels. This stain can detect as little as 50 pg of dsDNA. LiGreen™ Ultra is compatible with a standard 300 nm transilluminator, a 254 nm transilluminator, a blue-light transilluminator, or a gel reader equipped with visible light excitation such as a 488 nm laser-based gel scanner. LiGreen™ Ultra Nucleic Acid Gel Stain, 10,000× is a concentrated LiGreen™ Ultra solution that can be diluted 10,000 times for use in precast gel staining or 5,000 times for use in post gel staining according to the procedures described below. One vial of 10,000× solution can be used to prepare at least 100 precast minigels or post-stain at least 100 minigels.etc.
Gel staining with LiGreen™ Ultra is compatible with downstream applications such as gel extraction and cloning. LiGreen™ Ultra is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation.etc.
Key Features
• Safer than EB. Shown by Ames test and other tests to be non-mutagentic and non-cytotoxic.
• Stable at room temperature.
• Compatible with standard blue LED and UV transilluminators.
• Use with blue light reduces mutagenesis for gel excision and subsequent cloning.
Component
LiGreen™ Ultra Nucleic Acid Gel Stain (10,000× in DMSO): 500 μl
Storage
Store at 2-25°C and protect from light.
Case Study
Gel stain results with LiGreen™, LiGreen™ Plus, and LiGreen™ Ultra (Left), and comparison results with SYBR Safe and GelGreen (Right). Left: Two-fold serial dilutions of a 1 kb DNA ladder were loaded onto each gel in 3 lanes in the amounts of 25 ng, 50 ng and 100 ng (left to right), then post-stained with LiGreen™, LiGreen™ Plus, and LiGreen™ Ultra. Right: Results show LiGreen™ has similar sensitivity with SYBR Safe and GelGreen, LiGreen™ Plus and LiGreen™ Ultra have much better sensitivity.
