LiGreen™ Ultra Nucleic Acid Gel Stain (500 μl)

LiGreen™ Ultra Nucleic Acid Gel Stain is an ultra-sensitive green fluorescent stain, renowned for its ability to detect nucleic acids in agarose and polyacrylamide gels with exceptional precision. Capable of detecting as little as 50 picograms of double-stranded DNA, this stain sets a new standard in sensitivity. LiGreen™ Ultra is versatile in its compatibility, working seamlessly with a standard 300 nm transilluminator, a 254 nm transilluminator, a blue-light transilluminator, or a gel reader with visible light excitation, such as a 488 nm laser-based gel scanner.

The concentrated LiGreen™ Ultra Nucleic Acid Gel Stain, 10,000×, offers a highly potent solution that can be diluted up to 10,000 times for precast gel staining or 5,000 times for post-gel staining, following the procedures outlined below. A single vial of this 10,000× solution is sufficient to prepare at least 100 precast minigels or post-stain at least 100 minigels, providing a cost-effective and efficient solution for nucleic acid detection in various gel electrophoresis applications.

Gel staining with LiGreen™ Ultra is compatible with downstream applications such as gel extraction and cloning. LiGreen™ Ultra is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation.
 

Key Features

• Safer than EB. Shown by Ames test and other tests to be non-mutagentic and non-cytotoxic.

• Stable at room temperature.

• Compatible with standard blue LED and UV transilluminators.

• Use with blue light reduces mutagenesis for gel excision and subsequent cloning.
 

Component

1. LiGreen™ Ultra Nucleic Acid Gel Stain (10,000× in DMSO): 500 μl
 

Storage

Store at 2-25°C and protect from light.
 

Case Study

Gel stain results with LiGreen™, LiGreen™ Plus, and LiGreen™ Ultra (Left), and comparison results with SYBR Safe and GelGreen (Right). Left: Two-fold serial dilutions of a 1 kb DNA ladder were loaded onto each gel in 3 lanes in the amounts of 25 ng, 50 ng and 100 ng (left to right), then post-stained with LiGreen™, LiGreen™ Plus, and LiGreen™ Ultra. Right: Results show LiGreen™ has similar sensitivity with SYBR Safe and GelGreen, LiGreen™ Plus and LiGreen™ Ultra have much better sensitivity.