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The LiClone™ technology is simple, fast, and highly efficient DNA seamless cloning technology. It enables rapid directional cloning of inserts into any site in any vector. Use any method to linearize the vector, and introduce the end sequence of the linearized vector at the 5' end of the insert forward/reverse amplification primer, so that the 5' and 3' ends of the PCR product have the same ends sequence (15 - 20 bp) as the linearized vector, respectively. The PCR product with the same sequence as the end of the vector and the linearized vector are mixed in a certain proportion. Under the catalysis of recombinase, the transformation can be performed at 50°C for 5 - 30 min to complete the directional cloning.
LiClone™ Ultra One Step Cloning Kit is a new generation of recombinant cloning kits, compatible with 1 - 5 fragments homologous recombination. Highly optimized 2 × CE Mix further significantly improves the recombination efficiency of multiple fragments. This product has broader compatibility with the GC content of homology arms, which better guarantees the success rate of cloning in more difficult application scenarios.
Key Features
• Simple: Ready-to-use super mix in one tube.
• Fast: Recombination only takes 5 - 30 minutes.
• High-efficiency: Efficiently clone 50 bp - 10 kb fragments, the positive rate is > 95%.
• Compatible: Suitable for GC-rich/AT-rich homologous sequences.
• Special optimization: Maximum compatible with 15 - 35 bp overlaps, not only 15 - 20 bp
Applications
• Fast Cloning
• High-throughput Cloning
• Seamless Cloning
• Site-directed Mutagenesis
Components
1. 2× CE Mix: 100 μl
2. 500bp control insert (20 ng/μl): 5 μl
3. pUC19 control vector (50 ng/μl, Amp+): 5 μl
Storage
Store at -20°C.
