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| Applications: | WB, IHC, IF/IC, IP, ELISA |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Conjugation: | Unconjugated |
| Gene Name: | tubulin beta class I |
| Gene Symbol: | TUBB |
| Synonyms: | M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56; β-Tubulin |
| Gene ID: | 203068 |
| UniProt ID: | P07437 |
| Clone ID: | 1I8U1 |
| Immunogen: | Recombinant protein of human β-Tubulin |
| Dilution: | WB 1:5000-1:10000; IHC 1:50-1:200; IF/IC 1:50-1:200; IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
| Purification Method: | Affinity purification |
| Concentration: | 1 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 1% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Western blot analysis of various lysates using β-Tubulin Mouse mAb (AC021) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry analysis of paraffin-embedded Rat ovary using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat stomach using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunofluorescence analysis of C6 cells using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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