Cloning & Mutagensis Kits

The LiClone™ technology is simple, fast, and highly efficient DNA seamless cloning technology. It enables rapid directional cloning of inserts into any site in any vector. Use any method to linearize the vector, and introduce the end sequence of the linearized vector at the 5' end of the insert forward/reverse amplification primer, so that the 5' and 3' ends of the PCR product have the same ends sequence (15 - 20 bp) as the linearized vector, respectively. The PCR product with the same sequence as the end of the vector and the linearized vector are mixed in a certain proportion. Under the catalysis of recombinase, the transformation can be performed at 50°C for 5 - 30 min to complete the directional cloning.

LiClone™ Ultra One Step Cloning Kit is a new generation of recombinant cloning kit, compatible with 1 - 5 fragments homologous recombination. Highly optimized 2 × CE Mix further significantly improves the recombination efficiency of multiple fragments. This product has broader compatibility with the GC content of homology arms, which better guarantees the success rate of cloning in more difficult application scenarios.

Key Features

• Simple: Ready-to-use super mix in one tube.

• Rapid: saves 2-3 hours by eliminating vector digestion and gel purification steps.

• High-efficiency: Typically >95% of colonies bear the correctly inserted DNA fragment.

• Compatible: Suitable for GC-rich/AT-rich homologous sequences.

• Special optimization: Maximum compatible with 15 - 35 bp overlaps, not only 15 - 20 bp.
 

Product Name	Cat. #	Price	View
LiClone™ One Step Cloning Kit (25 rxns)	M0010	$149
LiClone™ Ultra One Step Cloning Kit (20 rxns)	M0011	$219
LiClone™ Fast Mutagenesis Kit (10 rxns)	M0012	$99
LiClone™ Fast DNA Ligation Kit (30 rxns)	M0009	$99

ExSembly™ Cloning Master Mix is a simple, fast, and high efficient product which enables directional insertion of any amplified DNA product into CIRCULAR vector. The ExSembly™ technology completely eliminates the time consuming step of preparing linear vector. The innovative buffer system in our master mix allows restriction enzymes to efficiently digest the circular DNA, and maintain high exonuclease and polymerase activity to enable the assembly to occur. This technology takes advantage of the fact that once the vector DNA successfully assembles with insert DNA, the restriction sites in the vector disappear. The restriction enzymes remove background negative clones by completely digesting unassembled vector DNA. One can conveniently use a relatively large amount of vector DNA, up to 500 ng, and obtain high number of positive clones, which consequently increase success rate.
 

Product Name	Cat. #	Price	View
2× ExSembly™ Cloning Master Mix (10 rxns)	M0005	$169

Publications

1. mRNA decapping activators Pat1 and Dhh1 regulate transcript abundance and translation to tune cellular responses to nutrient availability.
Publication: Nucleic Acids Research        Product: 2× ExSembly™ Cloning Master Mix

2. Genetic and enzymatic characterization of Amy13E from Cellvibrio japonicus reclassifies it as a cyclodextrinase also capable of α-diglucoside degradation.
Publication: Applied and Environmental Microbiology        Product: 2× ExSembly™ Cloning Master Mix

3. Galactomannan utilization by Cellvibrio japonicus relies on a single essential α-galactosidase encoded by the aga27A gene.
Publication: Molecular Microbiology        Product: 2× ExSembly™ Cloning Master Mix

Customer Testimonials

"I have been using the ExSembly™ Cloning Master Mix for two years and have had great success in cloning a wide range of DNA fragments using this mix. The product is extremely easy to use and makes cloning a very quick process. It is especially ideal to clone multiple DNA fragments simultaneously into a vector. Compared to other approaches and commercial kits I have used in the past; this product's efficiency is very impressive. I highly recommend the ExSembly™ Cloning Mix."

"ExSembly™ Cloning Master Mix works very well in my hands for almost two years. It has similar or higher efficiency compared with other assembly kits!"