Reactivity: | M,R & H,M,R |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Gene Name: | 5-hydroxytryptamine receptor 2A |
Synonyms: | 5-HT-2, 5-HT-2A, Serotonin receptor 2A, Htr2a, Htr2, serotonin 5-HT-2 receptor, 5 hydroxytryptamine receptor 2A |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse 5HT2A |
Uniprot ID: | P35363 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane |
Predicted MW. / Observed MW. | 53 kDa / 53 kDa |
Images
WB | Mouse, Rat | 1: 500-1: 1000 | cerebral cortex, brain, colon, cerebellum |
IHC/IF | Human, Mouse, Rat | 1: 200-1: 2000 | colon cancer, small intestine, colon |
Background
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. This receptor is involved in tracheal smooth muscle contraction, bronchoconstriction, and control of aldosterone production.
Images
Western blot analysis of 5HT2A (GB111001) at dilution of 1: 1000 | |
Immunohistochemistry analysis of paraffin embedded mouse small intestine using 5-HT2A (GB111001) at dilution of 1: 400 | |
Immunohistochemistry analysis of paraffin embedded human colon using 5-HT2A (GB111001) at dilution of 1: 400 | |
Immunohistochemistry analysis of paraffin embedded human colon cancer using 5-HT2A (GB111001) at dilution of 1: 400 | |
Immunofluorescent analysis of paraffin embedded rat colon using 5-HT2A (GB111001) at dilution of 1: 2000 | |
Immunofluorescent analysis of paraffin embedded mouse brain using 5-HT2A (GB111001) at dilution of 1: 2000 | |
Immunofluorescent analysis of paraffin embedded human colon using 5-HT2A (GB111001) at dilution of 1: 2000 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |