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| Product Category: | Human Adenovirus Type5 (dE1/E3) expressing Retinoblastoma 1 (including Osteosarcoma) under CMV promoter. |
| Description: | Retinoblastoma 1 (including Osteosarcoma), pre-made adenovirus, ready to ship and ready to use format. |
| Gene: | Retinoblastoma 1 (including Osteosarcoma) |
| NCBI Acc. #: | BC039060 |
| Gene ID: | 5925 |
| GI Number: | 24660139 |
| Fusion Tag: | C-terminus V5 epitope tag |
| Promoter: | CMV |
| Titer: | 1x1011~1x1012 PFU/ml |
| Storage Buffer: | DMEM with 2.5% BSA, 2.5% glycerol |
| Background: | Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. |
| Documents: | Please contact us to request the manual |
