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Anti-CD34 Rabbit pAb (100 μl)
| Reactivity: | M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Hematopoietic progenitor cell antigen CD34 |
Synonyms: | CD34, HPCA1, Mucosialin, Cluster designation 34, cluster of differentiation 34, CD34 antigen, CD34 molecule |
Immunogen: | Recombinant protein corresponding to Mouse CD34 |
Uniprot ID: | Q64314 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Membrane |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
IHC/IF | Mouse, Rat | 1: 300-1: 1800 | heart, kidney, liver, spleen, thymus |
Background
CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources.
Images
| Immunohistochemistry of paraffin embedded rat heart using CD34 (GB11013-1) at dilution of 1:300 (400x lens) |
| Immunohistochemistry of paraffin embedded rat kidney using CD34 (GB11013-1) at dilution of 1:300 (400x lens) |
| Immunofluorescence of paraffin embedded mouse liver using CD34 (GB11013-1) at dilution of 1:900 (400x lens) |
| Immunofluorescence of paraffin embedded mouse spleen using CD34 (GB11013-1) at dilution of 1:900 (400x lens) |
| Immunofluorescence of paraffin embedded rat thymus using CD34 (GB11013-1) at dilution of 1:900 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
