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Anti-BRCA1 Rabbit pAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Breast cancer type 1 susceptibility protein homolog |
Synonyms: | BRCA1, BRCAI, BRCC1, breast cancer 1, early onset, BROVCA1, IRIS, PSCP, RING finger protein 53, RNF53 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse BRCA1 |
Uniprot ID: | P48754 |
Isotype: | lgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Chromosome, Cytoplasm |
Product Usage Information
IHC/IF | Human, Mouse, Rat | 1: 100-1: 300 | colon, heart, lung, testis, pancreas |
Background
E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation.
Images
| IHC analysis of BRCA1 (GB115539). Sample: Human colon (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of BRCA1 (GB115539). Sample: Human lung cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of BRCA1 (GB115539). Sample: Human gastric cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of BRCA1 (GB115539). Sample: Human colon cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of BRCA1 (GB115539). Sample: Rat heart (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of BRCA1 (GB115539). Sample: Mouse heart (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of BRCA1 (GB115539). Sample: Human lung (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of BRCA1 (GB115539). Sample: Rat testis (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of BRCA1 (GB115539). Sample: Mouse testis (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of BRCA1 (GB115539). Sample: Mouse spleen (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of BRCA1 (GB115539). Sample: Rat pancreas (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
