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| Reactivity: | H,M & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Transcription activator BRG1 |
Synonyms: | SMARCA4, BAF190, BAF190A, BRG1, MRD16, RTPS2, SNF2, SNF2L4, SNF2LB, SWI2, hSNF2b, CSS4, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse BRG1 |
Uniprot ID: | Q3TKT4 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. / Observed MW. | 185 kDa / 185 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | lung |
IHC/IF | Human, Mouse, Rat | 1: 200-1: 800 | gall bladder, testis, stomach cancer |
Background
The protein is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin.
Images
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Western blot analysis of BRG-1 (GB11258) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin embedded rat testis using BRG-1 (GB11258) at dilution of 1: 200 |
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Immunohistochemistry analysis of paraffin embedded rat kidney using BRG-1 (GB11258) at dilution of 1: 200 |
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Immunohistochemistry analysis of paraffin embedded mouse testis using BRG-1 (GB11258) at dilution of 1: 200 |
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Immunohistochemistry analysis of paraffin embedded mouse kidney using BRG-1 (GB11258) at dilution of 1: 200 |
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Immunohistochemistry analysis of paraffin embedded human gall bladder using BRG-1 (GB11258) at dilution of 1: 400 |
| Immunohistochemistry analysis of paraffin embedded human tonsil using BRG-1 (GB11258) at dilution of 1: 400 |
| Immunofluorescent analysis of paraffin embedded rat lung using BRG-1 (GB11258) at dilution of 1: 400 |
| Immunofluorescent analysis of paraffin embedded rat hepatitis using BRG-1 (GB11258) at dilution of 1: 400 |
| Immunofluorescent analysis of paraffin embedded mouse nephritis using BRG-1 (GB11258) at dilution of 1: 400 |
| Immunofluorescent analysis of paraffin embedded mouse brain using BRG-1 (GB11258) at dilution of 1: 400 |
| Immunofluorescent analysis of paraffin embedded human stomach cancer using BRG-1 (GB11258) at dilution of 1: 400 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
