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| Reactivity: | H & H |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | HLA class II histocompatibility antigen gamma chain |
Synonyms: | CD74, DHLAG, HLADG, Ia GAMMA, Ii, p33, CLIP, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain |
Immunogen: | Recombinant human CD74 protein expressed in HEK293 cells |
Uniprot ID: | |
Isotype: | IgG1,κ |
Purity: | Affinity purification |
Subcellular location: | Cell membrane, Endosome, Lysosome, Golgi apparatus, Endoplasmic reticulum |
Predicted MW. / Observed MW. | 34 kDa / 34 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human | 1: 500-1: 1000 | Raji |
IHC/IF | Human | 1: 500-1: 1000 | hodgkin lymphoma, lung adenocarcinoma |
Background
CD74, also known as the MHC class II associated invariant chain (Ii), is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is produced in molar excess over MHC class II and some of this is expressed by an unknown pathway on the cell surface independent of or in association with MHC class II molecules.
Images
| Western blot analysis of CD74 (GB121179) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded human thodgkin lymphoma using CD74 (GB121179) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded human tonsil using CD74 (GB121179) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded human gastric carcinoma using CD74 (GB121179) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed human lung adenocarcinoma using CD74 (GB121179) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed human gastric adenocarcinoma using CD74 (GB121179) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed human tonsil using CD74 (GB121179) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
