Exosome & cfDNA Isolation Reagents

Exosome Isolation Reagents

Exosomes are small vesicles (50-120 nm in diameter) containing proteins, DNA/RNA and lipids, actively secreted by exocytosis in most living cells. LifeSct offers a wide selection of exosome isolation reagents from a wide variety of samples. The Exosome Isolation Reagent enables simple and reliable concentration of intact exosomes from different samples with a protocol that is scalable depending on your sample size.
 

Key Features

• Maximize recovery of intact exosomes for any type of downstream application.

• Easily isolate exosomes using our simple and reliable protocol.

• Avoid time-consuming ultra-centrifugation.

• Maximize flexibility: The reagents can be scaled up or down depending on the sample size.
 

Product Name	Cat. #	Price	View
Exosome Isolation Reagent from cell culture media (50 ml)	M0121	$249
Exosome Isolation Reagent from serum (10 ml)	M0122	$249
Exosome Isolation Reagent from plasma (10 ml)	M0123	$249
Exosome Isolation Reagent from urine (50 ml)	M0124	$249
Exosome Isolation Reagent from body fluids (10 ml)	M0134	$249

Exosome DNA Purification

LiPure™ Exosome DNA Purification Kits are designed for the isolation of exosomal DNA from pure exosome isolated by our Exosome Isolation Reagents. The kits also feature a buffer system that facilitates complete exosome lysis for efficient nucleic acid isolation. Exosomal DNA is bound to spin column or magnetic beads, washed and eluted. Normally, 100-300 ng of exosomal DNA can be purified from the exosome isolated from 200 μL blood serum or 5 mL cell media. The extracted DNA is ready for downstream applications such as sequencing, and PCR. No need to precipitate, concentrate or desalt.
 

Product Name	Cat. #	Price	View
LiPure™ Exosome DNA Purification Kit (25 rxns)	M0119	$199
LiPure™ Magbead Exosome DNA Purification Kit (25 rxns)	M0120	$199

cfDNA Purification

LiPure™ cfDNA Purification Kits provide a rapid and easy method for the isolation of circulating DNA from plasma, serum, and other cellular body fluids. The kits allow for simultaneous processing of single or multiple samples in under 2 hours. The isolated circulating DNA is ready for applications such as PCR, microarrays, and next generation sequencing.
 

Product Name	Cat. #	Price	View
LiPure™ cfDNA Purification Kit (50 rxns)	M0103	$599
LiPure™ Magbead cfDNA Purification Kit (50 rxns)	M0104	$599

Case Study

Electropherogram of purified cfDNA from serum sample using LiPure™ cfDNA Purification Kit from serum/plasma. Purified cfDNA was analyzed on Agilent’s 2100.

Plasma samples were purified using LiPure™ cfDNA Purification Kit from serum/plasma. The purified cfDNA samples were loaded on a D100 high-sensitive tape on TapeStation 2200.