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Anti-Cytokeratin 19 Rabbit pAb (100 μl)
| Reactivity: | M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Cytokeratin 19 rabbit polyclonal antibody |
Gene Name: | Keratin, type I cytoskeletal 19 |
Synonyms: | Cytokeratin-19, CK-19, Keratin-19, K19, Krt19, Krt1-19, 40 kDa keratin intermediate filament, K1CS |
Immunogen: | Recombinant protein corresponding to Mouse Cytokeratin 19 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane, Cytoplasm |
Predicted MW. | 45 kDa |
Observed MW. | 45 kDa |
Uniprot ID: | P08727, P19001, Q63279 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1: 500-1: 1000 | colon, lung |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 2000 | cholangiocarcinoma, colon, gastric cancer, kidney, skin, small intestine, stomach |
Background
Due to its high sensitivity, KRT19 is the most used marker for the RT-PCR-mediated detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients. Depending on the assays, KRT19 has been shown to be both a specific and a non-specific marker.
Images
| Western blot analysis of CK19 (GB112161) at dilution of 1: 500 |
| mmunohistochemistry of paraffin embedded human cholangiocarcinoma using CK19 (GB112161) at dilution of 1: 1500 (400x lens) |
| Immunohistochemistry of paraffin embedded human gastric cancer using CK19 (GB112161) at dilution of 1: 2000 (400x lens) |
| Immunohistochemistry of paraffin embedded human skin using CK19 (GB112161) at dilution of 1: 1500 (400x lens) |
| Immunohistochemistry of paraffin embedded human small intestine using CK19 (GB112161) at dilution of 1: 1500 (400x lens) |
| Immunohistochemistry of paraffin embedded mouse skin using CK19 (GB112161) at dilution of 1: 1500 (300x lens) |
| Immunohistochemistry of paraffin embedded mouse stomach using CK19 (GB112161) at dilution of 1: 1500 (400x lens) |
| Immunohistochemistry of paraffin embedded rat kidney using CK19 (GB112161) at dilution of 1: 1500 (400x lens) |
| Immunofluorescence of paraffin embedded human cholangiocarcinoma using CK19 (GB112161) at dilution of 1: 500 (400x lens) |
| Immunofluorescence of paraffin embedded human gastric cancer using CK19 (GB112161) at dilution of 1: 500 (400x lens) |
| Immunofluorescence of paraffin embedded mouse colon using CK19 (GB112161) at dilution of 1: 500 (400x lens) |
| Immunofluorescence of paraffin embedded mouse skin using CK19 (GB112161) at dilution of 1: 500 (400x lens) |
 | Immunofluorescence of paraffin embedded rat kidney using CK19 (GB112161) at dilution of 1: 500 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |