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Anti-Cytokeratin 5 Mouse mAb (100 μl)
| Reactivity: | H & H |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Keratin, type II cytoskeletal 5 |
Synonyms: | Cytokeratin-5, CK-5, Keratin-5, K5, Type-II keratin Kb5, Krt5, Krt2-5 |
Immunogen: | Recombinant protein corresponding to Mouse Cytokeratin 5 |
Uniprot ID: | Q922U2 |
Isotype: | IgG2b,κ |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Cell Membrane |
Predicted MW. / Observed MW. | 62 kDa / 62 kDa |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human | 1: 1000-1: 2000 | A431, HaCat, MCF-7 |
IHC/IF | Human | 1: 400-1: 800 | cervical cancer, esophagus, esophagus squamous-cell carcinoma, lung squamous-cell carcinoma |
Background
The protein encoded by this gene is a member of the keratin gene family. It is one type II keratin, is coexpressed with keratin 14 in stratified squamous epithelia. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex.
Images
| Western blot analysis of CK5 (GB121246) at dilution of 1: 2000 |
| Immunohistochemistry analysis of paraffin-embedded human cervical cancer using CK5 (GB121246) at dilution of 1: 800 |
| Immunohistochemistry analysis of paraffin-embedded human esophagus using CK5 (GB121246) at dilution of 1: 800 |
| Immunohistochemistry analysis of paraffin-embedded human esophagus squamous-cell carcinoma using CK5 (GB121246) at dilution of 1: 800 |
| Immunohistochemistry analysis of paraffin-embedded human lung squamous-cell carcinoma using CK5 (GB121246) at dilution of 1: 800 |
| Immunohistochemistry analysis of paraffin embedded human Non-small cell lung cancer using CK5 (GB121246) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded human Non-small cell lung cancer using CK5 (GB121246) at dilution of 1: 600 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
