Anti-ADGRE1 Rabbit pAb (100 μl)

F4/80 rabbit polyclonal antibody

Gene Name:

Adhesion G protein-coupled receptor E1

Synonyms:

ADGRE1, TM7LN3, EMR1, Gpf480, adhesion G protein-coupled receptor E1

Uniprot ID:

Q61549

Immunogen:

Recombinant protein corresponding to  Mouse F4/80

Isotype:

IgG

Purity:

Affinity purification

Subcellular location:

Cell membrane

Applications

Species

Dilution

Positive Sample

IHC/IF

Mouse

1: 500-1: 2000

liver, lung

IHC analysis of F480 (GB11027).
Sample: Mouse liver (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 1200, 4°C overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

IHC analysis of F480 (GB11027).
Sample: Mouse lung (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

IF analysis of F4-80 (GB11027).
Sample: Mouse liver+lps (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: TE buffer (pH 9.0) (G1203),98°C,20min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.