Reactivity: | M |
Applications: | WB |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | Fatty Acid Synthase rabbit polyclonal antibody |
Gene Name: | Fatty acid synthase |
Synonyms: | FASN, fatty acid synthase, Fasn,A630082H08Rik, FAS, OA-519, SDR27X1, Fatty acid synthase |
Immunogen: | Recombinant protein corresponding to Mouse Fatty acid synthase |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. | 273 kDa |
Observed MW. | 250-273 kDa |
Uniprot ID: | P19096, P12785 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse | 1: 500-1: 1000 | liver, lung |
IHC/IF | Mouse, Rat | 1: 500-1: 1200 | lung, spleen, testis |
Background
Fatty acid synthase is a multi-enzyme protein that catalyzes fatty acid synthesis. It is not a single enzyme but a whole enzymatic system composed of two identical 272 kDa multifunctional polypeptides, in which substrates are handed from one functional domain to the next. Its main function is to catalyze the synthesis of palmitate (C16:0, a long-chain saturated fatty acid) from acetyl-CoA and malonyl-CoA, in the presence of NADPH.
Images
Western blot analysis of FASN (GB11546) at dilution of 1: 500 | |
Immunohistochemistry of paraffin embedded mouse lung using FASN (GB11546) at dilution of 1: 600 (400x lens) | |
Immunohistochemistry of paraffin embedded rat lung using FASN (GB11546) at dilution of 1: 600 (400x lens) |
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Immunofluorescence of paraffin embedded mouse testis using FASN (GB11546) at dilution of 1: 500 (400x lens) |
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Immunofluorescence of paraffin embedded rat spleen using FASN (GB11546) at dilution of 1: 500 (400x lens) |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |