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Anti-GTF2F2 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & M,R & M,R |
| Applications: | WB & IHC & IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | GTF2F2 rabbit polyclonal |
Gene Name: | General transcription factor IIF subunit 2 |
Synonyms: | Gtf2f2, GTF2F2, BTF4, RAP30, TF2F2, TFIIF, general transcription factor IIF subunit 2 |
Immunogen: | Recombinant protein corresponding to Mouse GTF2F2 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. | 28 kDa |
Observed MW. | 30 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | spleen, lung, testis |
IHC | Mouse, Rat | 1: 1500-1: 4000 | testis, lung, brain |
IF | Mouse, Rat | 1: 1000-1: 2000 | testis, lung, brain |
Background
TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity.
Images
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Western blot analysis of GTF2F2 (GB11928) at dilution of 1: 1000 Lane 1: PC3 cell lysate Lane 2: Mouse spleen tissue lysate Lane 3: Mouse lung tissue lysate Lane 4: Mouse testis tissue lysate Lane 5: Rat spleen tissue lysate Lane 6: Rat lung tissue lysate Lane 7: Rat testis tissue lysate |
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Immunohistochemistry analysis of paraffin-embedded mouse testis using GTF2F2 (GB11928)at dilution of 1: 3000 |
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Immunohistochemistry analysis of paraffin-embedded rat lung using GTF2F2 (GB11928) at dilution of 1: 3000 |
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Immunohistochemistry analysis of paraffin-embedded mouse brain using GTF2F2 (GB11928) at dilution of 1: 3000 |
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Immunohistochemistry analysis of paraffin-embedded rat brain using GTF2F2 (GB11928) at dilution of 1: 3000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
