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Anti-HERC4 Rabbit pAb (100 μl)
| Reactivity: | R & M,R & M |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Probable E3 ubiquitin-protein ligase HERC4 |
Synonyms: | hect domain and RLD 4, KIAA1593 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse HERC4 |
Uniprot ID: | Q6PAV2 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. / Observed MW. | 118 kDa / 118 kDa |
Product Usage Information
WB | Rat | 1: 800-1: 1500 | testis, placenta |
IHC/IF | Mouse, Rat | 1: 800-1: 2400 | heart, spleen |
ICC/IF | Mouse | 1: 100-1: 400 | NIH-3T3 |
Background
Probable E3 ubiquitin-protein ligase involved in either protein trafficking or in the distribution of cellular structures. Required for spermatozoon maturation and fertility, and for the removal of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer it to targeted substrates.
Images
| Western blot analysis of HERC4 (GB114906) at dilution of 1: 800 |
| Immunohistochemistry of paraffin embedded rat hert using HERC4 (GB114906) at dilution of 1: 800 |
| Immunohistochemistry of paraffin embedded mouse spleen using HERC4 (GB114906) at dilution of 1: 800 |
| Immunohistochemistry of paraffin embedded rat heart using HERC4 (GB114906) at dilution of 1: 800 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH-3T3 cells using HERC4 (GB114906) at dilution of 1: 200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
