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| Reactivity: | H,M |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | IL-10 rabbit polyclonal |
Gene Name: | Interleukin-10 |
Synonyms: | IL10, CSIF, GVHDS, IL-10, IL10A, TGIF, interleukin 10, Cytokine Synthesis Inhibitory Factor, Interleukin-10 precursor |
Immunogen: | Recombinant protein corresponding to Mouse IL-10 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Secreted |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
IHC | Mouse, Rat | 1: 200-1: 1000 | inflammatory lung, inflammatory liver, inflammatory spleen |
IF | Mouse, Rat | 1: 200-1: 1000 | inflammatory lung, inflammatory liver, inflammatory spleen |
Background
IL-10 is a cytokine with multiple, pleiotropic, effects in immunoregulation and inflammation. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10 is capable of inhibiting synthesis of pro-inflammatory cytokines such as IFN-γ, IL-2, IL-3, TNFα and GM-CSF made by cells such as macrophages and Th1 T cells. It also displays a potent ability to suppress the antigen-presentation capacity of antigen presenting cells. IL-10 is linked to the myokines, as exercise provokes an increase in circulating levels of IL-1ra, IL-10, and sTNF-R, suggesting that physical exercise fosters an environment of anti-inflammatory cytokines.
Images
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Immunohistochemistry analysis of paraffin-embedded mouse inflammatory lung using IL-10 (GB11534) at dilution of 1: 500 |
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Immunohistochemistry analysis of paraffin-embedded rat inflammatory lung using IL-10 (GB11534) at dilution of 1: 500 |
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Immunohistochemistry analysis of paraffin-embedded rat inflammatory spleen using IL-10 (GB11534) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |