Reactivity: | M,R & H,R & H,M |
Applications: | WB & IHC/IF & ICC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Gene Name: | Isovaleryl-CoA dehydrogenase, mitochondrial |
Synonyms: | IVD, ACAD2, Butyryl-CoA dehydrogenase |
Immunogen: | Recombinant protein corresponding to Mouse IVD |
Uniprot ID: | Q9JHI5 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Mitochondrion |
Predicted MW. / Observed MW. | 46 kDa / 46 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 1000-1: 2000 | kidney, heart, liver |
IHC/IF | Human, Rat | 1: 1400-1: 2800 | kidney, pancreas |
ICC/IF | Human, Mouse | 1: 50-1: 200 | HeLa |
Background
IVD(Isovaleryl CoA dehydrogenase, mitochondrial) is a member of the acylCoA dehydrogenase family and is involved in the catabolism of leucine. In size, IVD precursor and mature proteins produced by class I mutants are indistinguishable from their normal counterparts. Class II, III, and IV mutants make IVD precursor proteins 42 kD in size rather than the normal 45 kD. It has 2 isoforms produced by alternative splicing.
Images
WB analysis of IVD (GB113133). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. | |
WB analysis of IVD (GB113133). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. | |
IHC analysis of IVD (GB113133). Sample: Human kidney , 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 2800, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. | |
IHC analysis of IVD (GB113133). Sample: Rat kidney , 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 2800, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. | |
IHC analysis of IVD (GB113133). Sample: Rat pancreas , 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 2800, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. | |
ICC analysis of IVD (GB113133)green. Sample: NIH3T3, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 50, 4°C overnight. Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h.. | |
ICC analysis of IVD (GB113133)green. Sample: Hela, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 50, 4°C overnight. Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h.. |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |