Anti-IVD Rabbit pAb (100 μl)

Anti-IVD Rabbit pAb (100 μl)

Brand: ServiceBio
Cat. #: GB113133-100
Availability: In Stock
$259.00
-+
Reactivity: M,R & H,R & H,M    
Applications: WB & IHC/IF & ICC/IF    
Host Species: Rabbit    
Clonality: Polyclonal    
Gene Name:

Isovaleryl-CoA dehydrogenase, mitochondrial

Synonyms:

IVD, ACAD2, Butyryl-CoA dehydrogenase

Immunogen:

Recombinant protein corresponding to Mouse IVD

Uniprot ID:

Q9JHI5

Isotype:

IgG

Purity:

Affinity purification

Subcellular location:

Mitochondrion

Predicted MW. / Observed MW.

46 kDa / 46 kDa




Product Usage Information

WB

Mouse, Rat

1: 1000-1: 2000

kidney, heart, liver

IHC/IF

Human, Rat

1: 1400-1: 2800

kidney, pancreas

ICC/IF

Human, Mouse

1: 50-1: 200

HeLa




Background

IVD(Isovaleryl CoA dehydrogenase, mitochondrial) is a member of the acylCoA dehydrogenase family and is involved in the catabolism of leucine. In size, IVD precursor and mature proteins produced by class I mutants are indistinguishable from their normal counterparts. Class II, III, and IV mutants make IVD precursor proteins 42 kD in size rather than the normal 45 kD. It has 2 isoforms produced by alternative splicing.




Images

01  GB113133_9463 IVD_WB-1.png

WB analysis of IVD (GB113133).

Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Primary antibody: 1: 1000, 4°C overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h.

02  GB113133_9463 IVD_WB-2.png

WB analysis of IVD (GB113133).

Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Primary antibody: 1: 1000, 4°C overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h.

03  GB113133_20210410_IVD_9463_946320122302 H kidney d2800_28.8x_IHC.png

IHC analysis of IVD (GB113133).

Sample: Human kidney , 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.

Primary antibody: 1: 2800, 4°C overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04  GB113133_20210410_IVD_9463_946320122302 R kidney d2800_40.1x_IHC.png

IHC analysis of IVD (GB113133).

Sample: Rat kidney , 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.

Primary antibody: 1: 2800, 4°C overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

05  GB113133_20210410_IVD_9463_946320122302 R pancreas d2800_31.8x_IHC.png

IHC analysis of IVD (GB113133).

Sample: Rat pancreas , 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.

Primary antibody: 1: 2800, 4°C overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

06 GB113133_IVD_green_d50_NIH-3T3_20x_ICC.png

ICC analysis of IVD (GB113133)green.

Sample: NIH3T3, 4% PFA (G1101) 20min.

Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.

Primary antibody: 1: 50, 4°C overnight.

Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h..

07 GB113133 IVD_green_d50_HeLa_20x_ICC.png

ICC analysis of IVD (GB113133)green.

Sample: Hela, 4% PFA (G1101) 20min.

Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.

Primary antibody: 1: 50, 4°C overnight.

Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h..




Storage

StorageStore at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage BufferPBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


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