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| Reactivity: | Human |
| Applications: | WB, IP |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | acylaminoacyl-peptide hydrolase |
| Gene Symbol: | APEH |
| Synonyms: | APH; OPH; AARE; ACPH; D3S48E; D3F15S2; DNF15S2; EH |
| Gene ID: | 327 |
| UniProt ID: | P13798 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human APEH (NP_001631.3). |
| Dilution: | WB 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.71 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-APEH polyclonal antibody |
Background
This gene APEH encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma.
Images
 | Western blot analysis of various lysates, using [KO Validated] APEH Rabbit pAb (A5893) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of lysates from wild type(WT) and APEH knockout (KO) 293T(KO) cells, using [KO Validated] APEH Rabbit pAb (A5893) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunoprecipitation analysis of 100 μg extracts of SW480 cells using 3 μg APEH antibody (A5893). Western blot was performed from the immunoprecipitate using APEH antibody (A5893) at a dilution of 1:1000. |
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