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Anti-Lamin B1 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R & H,M,R |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Lamin-B1 |
Synonyms: | LMNB1, LMN2, LMNB, ADLD, LMN |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Lamin B1 |
Uniprot ID: | P14733 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. / Observed MW. | 66 kDa / 69 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | spleen |
IHC/IF | Human, Mouse, Rat | 1: 300-1: 800 | bladder cancer, brain, breast cancer |
ICC/IF | Human, Mouse, Rat | 1: 100-1: 400 | HeLa |
Background
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane.
Images
| Western blot analysis of Lamin-B1 (GB111802) at dilution of 1: 500 |
| Immunohistochemistry of paraffin embedded human colon using LaminB1 (GB111802) at dilution of 1: 300 (400x lens) |
| Immunohistochemistry of paraffin embedded human ovarian cancer using LaminB1 (GB111802) at dilution of 1: 300 (400x lens) |
| Immunohistochemistry of paraffin embedded mouse placenta using LaminB1 (GB111802) at dilution of 1: 300 (400x lens) |
| Immunohistochemistry of paraffin embedded rat brain using LaminB1 (GB111802) at dilution of 1: 300 (400x lens) |
| Immunohistochemistry of paraffin embedded rat heart using LaminB1 (GB111802) at dilution of 1: 300 (400x lens) |
| Immunohistochemistry of paraffin embedded rat placenta using LaminB1 (GB111802) at dilution of 1: 300 (400x lens) |
| Immunofluorescence of paraffin embedded human bladder cancer using LaminB1 (GB111802) at dilution of 1: 300 (400x lens) |
| Immunofluorescence of paraffin embedded human breast cancer using LaminB1 (GB111802) at dilution of 1: 300 (400x lens) |
| Immunofluorescence of paraffin embedded human metrocarcinoma using LaminB1 (GB111802) at dilution of 1: 300 (400x lens) |
| Immunofluorescence of paraffin embedded mouse brain using LaminB1 (GB111802) at dilution of 1: 300 (400x lens) |
| Immunofluorescence of paraffin embedded mouse heart using LaminB1 (GB111802) at dilution of 1: 300 (400x lens) |
| Immunofluorescence of paraffin embedded rat placenta using LaminB1 (GB111802) at dilution of 1: 300 (400x lens) |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using LaminB1 (GB111802) at dilution of 1: 200 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using LaminB1 (GB111802) at dilution of 1: 200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
