LiQuant™ Green qPCR Master Mix utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR Green I based quantitative PCR (qPCR). LiQuant™ Probe qPCR Master Mix is a special reagent designed for probe based qPCR. Utilizing hot-start LiQuant™ DNA Polymerase, alone with optimal buffer specially optimized for qPCR, it is capable of significant restraining non-specific amplification as well as enhancing the amplification efficiency.
Selection Guide
Product Name | Type | Sensitivity | Specificity |
LiQuant™ Universal | SYBR green | +++++ | ++++ |
LiQuant™ Ultra | SYBR green | +++++ | +++++ |
LiQuant™ Probe | Probe-based | +++++ | +++++ |
Key Features
• The qPCR mixes are a 2× premixed reagent which is convenient to handle.
• The mixes can be directly used for robust and low-template quantitative PCR with high sensitivity and specificity.
• High reliability: the mixes are stable at room temperature in one week.
Case Study
Publications
1. YAP/TEAD1 Complex Is a Default Repressor of Cardiac Toll-Like Receptor Genes.
Publication: International Journal of Molecular Sciences Product: LiQuant™ Universal Green qPCR Master Mix
2. Knockdown of lncRNA TP53TG1 Enhances the Efficacy of Sorafenib in Human Hepatocellular Carcinoma Cells.
Publication: non-coding RNA Product: LiQuant™ Universal Green qPCR Master Mix
3. Selectively expressing SARS-CoV-2 Spike protein S1 subunit in cardiomyocytes induces cardiac hypertrophy in mice.
Publication: bioRxiv Product: LiQuant™ Universal Green qPCR Master Mix
Customer Testimonials
"We were pleasantly surprised at how well the LiQuant™ Universal Green qPCR Mater Mix worked with our typically problematic samples. I was not familiar with LifeSct prior to this but will definitely keep them in mind for future molecular biology needs and would recommend this product."
"We have tested the LiQuant™ Universal Green qPCR Mater Mix. It worked pretty well and I believe we will order it soon. Thank you!"
"I have been using LiTaq™ Plus PCR Master Mix and LiQuant™ Universal Green qPCR Master Mix in my experiments for two years. The PCR mix can impressively amplify the difficult sequences in my hands. Amazingly, it rapidly amplifies the templates at ~ 2kb/min. And the qPCR mix has the higher sensitivity compared with the similar product from other company. I highly recommend these products!!!"