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| Reactivity: | H,M,R & H |
| Applications: | WB & IHC |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | MID1 rabbit polyclonal |
Gene Name: | E3 ubiquitin-protein ligase Midline-1 |
Synonyms: | Midin, RING finger protein Midline-1, RING-type E3 ubiquitin transferase Midline-1, Tripartite motif-containing protein 18, Mid1, Fxy, Trim18 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse MID1 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Cytoskeleton |
Predicted MW. | 76 kDa |
Observed MW. | 76 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 2000-1: 3000 | heart, brain, testis, liver |
IHC | Human | 1: 600-1: 1200 | tonsil |
Background
Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.
Images
| Western blot analysis of MID1 (GB112569) at dilution of 1: 3000 Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: K562 cell lysate Lane 4: Mouse heart tissue lysate Lane 5: Mouse brain tissue lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat heart tissue lysate Lane 8: Rat brain tissue lysate Lane 9: Rat testis tissue lysate |
| Western blot analysis of MID1 (GB112569) at dilution of 1: 3000 Lane 1: HeLa cell lysate Lane 2: HUVEC cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF7 cell lysate Lane 5: HEK293 cell lysate Lane 6: Mouse brain tissue lysate Lane 7: Mouse liver tissue lysate Lane 8: Rat brain tissue lysate Lane 9: Rat liver tissue lysate |
| Immunohistochemistry analysis of paraffin-embedded human tonsil using MID1 (GB112569) at dilution of 1: 1200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |