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| Reactivity: | H |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Stromelysin-1 |
Synonyms: | MMP3, CHDS6, MMP-3, SL-1, STMY STMY1, STR1, matrix metallopeptidase 3 |
Immunogen: | Recombinant protein corresponding to Mouse MMP3 |
Uniprot ID: | P28862 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Extracellular matrix |
Predicted MW. / Observed MW. | 54 kDa / 47 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | lung, spleen |
IHC/IF | Mouse, Rat | 1: 400-1: 1600 | pneumonia, splenitis |
Background
Metalloproteinase with a rather broad substrate specificity that can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates different molecules including growth factors, plasminogen or other matrix metalloproteinases such as MMP9. Once released into the extracellular matrix (ECM), the inactive pro-enzyme is activated by the plasmin cascade signaling pathway. Acts also intracellularly. For example, in dopaminergic neurons, gets activated by the serine protease HTRA2 upon stress and plays a pivotal role in DA neuronal degeneration by mediating microglial activation and alpha-synuclein/SNCA cleavage. In addition, plays a role in immune response and possesses antiviral activity against various viruses. Mechanistically, translocates from the cytoplasm into the cell nucleus upon virus infection to influence NF-kappa-B activities.
Images
| WB analysis of MMP3(GB11131). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| WB analysis of MMP3(GB11131). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
 | IHC analysis of MMP3 (GB11131). Sample: Mouse pneumonia, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Primary antibody: 1: 400, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of MMP3 (GB11131). Sample: Rat splenitis, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Primary antibody: 1: 400, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
