NGS Library Preparation Reagents

DNA Library Preparation

VAHTS Universal DNA Library Prep Kit for Illumina V4 is a library preparation kit targeted for Illumina high-throughput sequencing platform optimization. 100 pg -1 μg of fragmented double-stranded DNA can be converted into a dedicated library for Illumina high-throughput sequencing platform. As a new generation of upgraded version, the kit can improve the conversion rate of low-quality template libraries and reduce the repetition rate of libraries by optimizing and improving the terminal repair module, connecting module and library amplification module. This kit is widely suitable for PCR or PCR-Free library preparation of a variety of samples, and is compatible with the targeted capture process. All reagents provided in the kit have undergone strict quality control and functional validation to ensure the stability and repeatability of library preparation to the greatest extent.
 

Key Features

• Wide Compatibility: Compatible with FFPE and cfDNA samples of low quality in addition to conventional samples.

• High Efficiency: Higher library conversion rate.

• Accurate Detection: Accurate detection of low-frequency mutations.
 

Product Name	Cat. #	Price	View
VAHTS Universal DNA Library Prep Kit for Illumina V4 (24 rxns)	ND610	$579
VAHTS Universal Plus DNA Library Prep Kit for Illumina V2 (24 rxns)	ND627	$619

RNA Library Preparation

VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina is an RNA-seq library preparation kit specifically designed for Illumina high-throughput sequencing platforms. The kit is perfectly compatible with total RNA from various types of samples, and the minimum starting input required for this kit is as low as 10 ng.In addition, this kit combines second-strand synthesis, end repair, and dA-tailing in a single step and eliminates the need for purification during the process, which greatly streamlines the operation workflow and shortens the library preparation time. The kit also provides two types of cDNA Second- Strand Synthesis Buffer for either general or strand-specific RNA-seq library preparation.
 

Product Name	Cat. #	Price	View
VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina (24 rxns)	NR605	$839

Epigenetics

EpiArt DNA Methylation Library Kit for Illumina V3 for Illumina is a methyl-seq DNA library preparation kit that was specifically developed for the Illumina high-throughput sequencing platform. Based on high-efficiency single strand ligation technology, this kit can convert 10 pg - 250 ng of converted DNA into a sequencing library. It is compatible with DNA samples as short as 40 bp. The optimized library preparation process enables the library preparation to be completed within 2 h, and the manual operation duration is less than 30 min. It is suitable for automatic library preparation and compatible with the capture process. All the reagents in the kit have undergone rigorous quality control and function testing to ensure the optimal stability and repeatability of library preparation.
 

Product Name	Cat. #	Price	View
EpiArt DNA Methylation Library Kit for Illumina V3 (24 rxns)	NE103	$1559

Magnetic Beads

LiPure™ NGS Library Prep Beads are used for nucleic acid size selection in the library construction process for Next Generation Sequencing (NGS). LiPure™ NGS Library Prep Beads are uniform and stable with high binding capacity, and slow sedimentation. The Beads are suspended in an optimized binding buffer that facilitates the ratio-metric size selection of NGS DNA/RNA library.
 

Product Name	Cat. #	Price	View
LiPure™ NGS Library Prep Beads (5 ml)	M0131	$269
LiPure™ Streptavidin Magnetic Beads (1 ml)	M0129	$99

NGS Library Quantification

LiQuant™ NGS dsDNA Assay Kit is designed specifically for an easy and accurate quantitation of dsDNA. The kit is highly selective for dsDNA over RNA, and offers advantages in stability, linear dynamic range, and sensitivity over other traditional of DNA quantitation. The kit is designed in an "Add-and-Read" format to simplify DNA quantitation and speed up your workflow. The kit is ideal for DNA quantitation in NGS library. The assay is well tolerated to common contaminants such as proteins, salts, solvents, detergents, or free nucleotides. The assay can be adapted for use in microplates, tubes or cuvettes.
 

Product Name	Cat. #	Price	View
LiQuant™ NGS dsDNA Assay Kit (200 rxns)	M0066	$129

Case Study

Suitable for low-quality FFPE, cfDNA. Different input amounts of clinical FFPE and cfDNA were used as templates for library construction. Under the same number of amplification cycles, the ND610 library output had significant advantages over similar products of Supplier A.

Higher library conversion rate. Different samples and different input amounts were used for library construction. Under different library conversion rate evaluation methods, the library conversion rate of ND610 was significantly better than similar products of Supplier A.

Higher effective sequencing depth. The library was constructed using different standards and clinical samples of different qualities as templates. After the sequencing data was normalized, the sequencing depth of the library constructed by ND610 was higher after deduplication.
 

Publications

1. SALL2 regulates neural differentiation of mouse embryonic stem cells through Tuba1a. 

Publication: Cell Death & Disease        Product: VAHTS Universal DNA Library Prep Kit for Illumina V4

2. Effects of calcium phosphate and phosphorus-dissolving bacteria on microbial structure and function during Torreya Grandis branch waste composting. 
Publication: BMC Microbiology        Product: VAHTS Universal Plus DNA Library Prep Kit for Illumina V2

3. NAT10-mediated mRNA N 4 -acetylcytidine reprograms serine metabolism to drive leukaemogenesis and stemness in acute myeloid leukaemia. 

Publication: Nature cell biology        Product: VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina

4. The gonadal niche safeguards human fetal germline cell development following maternal SARS-CoV-2 infection. 

Publication: Cell Reports        Product: EpiArt DNA Methylation Library Kit for Illumina V3