Your shopping cart is empty!
| Reactivity: | H |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Receptor-interacting serine/threonine-protein kinase 2 |
Synonyms: | CARD3, CARDIAK, CCK, GIG30, Receptor interacting protein 2, RICK, RIP 2, RIP2, RIPK2 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse RICK |
Uniprot ID: | P58801 |
Isotype: | lgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 60 kDa / 46-55,61-72 kDa |
Product Usage Information
WB | Human | 1: 300-1: 500 | Hela, A431, K562 |
Background
Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Once recruited, autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is release from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis.
Images
| WB analysis of RICK (GB115502). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 300, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
